Restriction fragment length polymorphisms as markers of engraftment in allogeneic marrow transplantation
نویسندگان
چکیده
منابع مشابه
Restriction fragment length polymorphisms as markers of engraftment in allogeneic marrow transplantation.
We have used DNA hybridization techniques employing restriction fragment length polymorphisms (RFLPs) to quantitate the level of donor cell engraftment in bone marrow transplantation recipients. The genetic origin of the bone marrow cells and various peripheral blood populations was analyzed in 14 patients. We found at least one informative polymorphism for each donor-recipient pair. Additional...
متن کاملDeterminants of engraftment after allogeneic marrow transplantation.
ESPITE RECENT improvements in posttransplant D immunosuppressive regimens, acute and chronic graftversus-host disease (GVHD) remain significant obstacles to successful marrow transplantation, particularly in patients not having an HLA-identical related donor. These findings have lead to continued interest in preventing GVHD by depleting T cells from the donor marrow. Initial animal experiments ...
متن کاملMan Using Restriction Fragment Length Polymorphisms
We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted...
متن کاملNeisseria gonorrhoeae by restriction fragment length polymorphisms
Objective-To characterise Neisseria gonorrhoeae isolates by restriction fragment length polymorphisms (RFLPs) in ribosomal RNA genes. Design-Generation of RFLP patterns by HincII restriction of rRNA genes followed by hybridisation with a nonradioactive labelled broad spectrum 16 + 23S rRNA gene probe. This typing method was developed and compared with MAb based serotyping. Specimens-Forty three...
متن کاملInheritance of restriction fragment length polymorphisms in Agaricus brunnescens.
The cultivated mushroom, Agaricus brunnescens, is secondarily homothallic; most basidia produce only two basidiospores, each of which receives two of the four post meiotic nuclei. The segregation of restriction fragment length polymorphisms (RFLPs) detected by four plasmid probes carrying single-copy nuclear DNA of Agaricus was followed in seven parental strains including commercial, wild-colle...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 1985
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v66.6.1436.bloodjournal6661436